Comparative Study of the Electrochemical Signal of Neonicotinoids and Tetronic Acid Amides on Screen Printed Electrodes With and Without the Use Of N2a Cells.
Please use any of these identifiers to cite or link to this item: http://hdl.handle.net/10795/2728 http://repository.edulll.gr/2728
The extensive use of pesticides in agriculture has caused significant concern in public health therefore cell-based sensors have been proved as potentially useful method for studying their effects. The objective of this work was to investigate the possibility of using carbon screen printed electrodes (SPE) in combination with the use of N2a cells for the direct voltammetric determination of 5 neonicotinoids (imidacloprid, clothianidin, thiacloprid, acetamiprid and thiamethoxam) and 3 tetronic acid amide insecticides (spiromesifen, spirodiclofen and spirotetramat). The insecticide cytotoxicity in N2a cells was determined after 30 min treatment with concentrations 3, 10, 30 and 100 μM by the propidium iodide (PI) uptake assay. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were performed to compare signals from plain carbon screen printed electrodes and from N2a cells.
